Immunoglobulin G ELISA (IgG)

The Immunoglobulin G ELISA (IgG) is intended for the quantitative determination of IgG in plasma, serum, and urine.

For research use only. Not for use in diagnostic procedures.

For Laboratory Professional Use Only.

This is the RUO version of Immundiagnostik part number K 6510A.

Test Principle of the Immunoglobulin G ELISA

The Immunoglobulin G ELISA is designed for the quantitative determination of IgG in plasma, serum, and urine.

In the first incubation step, the Immunoglobulin G in the samples is bound to polyclonal rabbit antibodies (in excess) immobilized to the surface of the microtiter wells. After removing all unbound substances, a peroxidase-labeled anti-immunoglobulin G antibody is added. The second washing step is followed by incubation with the substrate, tetramethylbenzidine (TMB). The reaction is terminated by an acidic stop solution converting the color from blue to yellow. The intensity of the yellow color is directly proportional to the concentration of Immunoglobulin G in the sample.

A dose-response curve of the absorbance unit (optical density, OD at 450 nm) vs. concentration is generated using the results obtained from the calibrators. Immunoglobulin G, present in the samples, is determined directly from this curve.