The IDKmonitor® Adalimumab Total ADA ELISA (Health Canada) is intended for the determination of human antibodies against TNFα blocker adalimumab (e.g. HUMIRA®) in the presence of adalimumab in EDTA plasma and serum.
Health Canada Licensed. For Laboratory Professional Use Only.
Not for sale in the U.S. Please contact us to order or get a quote.
If you are in the U.S., you can purchase the RUO version of the Adalimumab Total ADA ELISA.
Browse our other IDKmonitor® Adalimumab ELISAs.
Download our IDKmonitor® ELISAs brochure to discover more.
The IDKmonitor® Adalimumab Total ADA ELISA (Health Canada) is designed for the quantitative determination of antibodies against TNFα blocker adalimumab (e. g. HUMIRA®).
During sample preparation, the anti-drug antibodies (ADA) are separated from the therapeutic antibody in order to acquire free ADA. By adding the conjugate (peroxidase-labeled therapeutic antibody) and the tracer (biotinylated therapeutic antibody), the unmarked therapeutic antibodies are replaced, and the marked antibodies can form a complex with the ADA. This complex binds via biotin to the streptavidin-coated microtiter plate. It is detected via the peroxidase conjugate with the peroxidase converting the substrate TMB to a blue product.
The enzymatic reaction is stopped by adding an acidic solution. The samples convert from blue to yellow. The color change should be measured in a photometer at 450 nm. The interpretation is made using the cut-off control.
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