The IDK® Secretory IgA ELISA (sIgA) is an enzyme immunoassay intended for the quantitative determination of secretory IgA (sIgA) in saliva and stool.
Health Canada Licensed. Not for sale in the U.S. Please contact us for a quote.
For Laboratory Professional Use Only.
The IDK® Secretory IgA ELISA (sIgA) is also available in a customized bulk pack (20 plates) if your lab is testing at high volumes.
Developed by Immundiagnostik AG.
In the first incubation step, the sIgA in the samples is bound to polyclonal antibodies (rabbit anti-human IgA), which are immobilized to the surface of the microtiter wells. To remove all unbound substances, a washing step is carried out.
In the second incubation step, a peroxidase-labeled conjugate (mouse anti-sIgA) is added, specifically recognizing the bound secretory IgA. After another washing step to remove all unbound substances, the solid phase is incubated with a tetramethylbenzidine (TMB) substrate. An acidic stop solution is then added to stop the reaction. The color converts from blue to yellow. The intensity of the yellow color is directly proportional to the concentration of secretory IgA.
A dose-response curve of the absorbance unit (optical density, OD) vs. concentration is generated using the results obtained from the standards. The presence of secretory IgA in the patient samples is determined directly from this curve.
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