IDK® H. Pylori Antigen ELISA

The IDK® H. Pylori Antigen ELISA is a sandwich ELISA for the determination of Helicobacter pylori in stool.

For research use only in the U.S. Not for use in diagnostic procedures.

For Laboratory Professional Use Only.

This is the RUO version of Immundiagnostik part number K 6923.

Developed by Immundiagnostik AG.

Resources

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Test Principle of the IDK® H. Pylori Antigen ELISA

A microtiter plate is coated with antibodies specific to Helicobacter pylori. The immobilized antibodies bind the Helicobacter pylori antigen in the sample during the first incubation step. At the same time, a peroxidase-labeled antibody binds the Helicobacter pylori antigen. After a washing step, tetramethylbenzidine is used as a substrate for peroxidase.

Finally, an acidic stop solution is added to terminate the reaction. The color changes from blue to yellow. The intensity of the color is proportional to the amount of analyte (sample or control). The results are evaluated by comparison with a cut-off value.