IDK® Gluten Fecal ELISA (Quantitative)

The IDK® Gluten Fecal ELISA test is intended for the quantitative determination of Gliadin 33mer in stool samples.

For Research Use Only. Not for use in diagnostic procedures.

For Laboratory Professional Use Only.

Developed by Immundiagnostik AG.

Test Principle of the IDK® Gluten Fecal ELISA

The IDK® Gluten Fecal ELISA is designed for the quantitative determination of gliadin 33mer. The assay utilizes the sandwich technique with two selected polyclonal antibodies that bind to gliadin-33mer.

Standards, controls, and extracted samples that are assayed for gliadin 33mer are added into the wells of a microplate coated with a high affine polyclonal gliadin 33mer antibody.

During the first incubation step, gliadin 33mer is bound by the immobilized antibody. Then a peroxidase-conjugated polyclonal gliadin 33mer antibody is added into each microtiter well, and a sandwich of capture antibody – gliadin 33mer – peroxidase-conjugate is formed. Tetramethylbenzidine is used as peroxidase substrate. Finally, an acidic stop solution is added to terminate the reaction.

The color changes from blue to yellow. The intensity of the yellow color is directly proportional to the concentration of gliadin 33mer. A dose-response curve of the absorbance unit (optical density, OD at 450 nm) vs. concentration is generated, using the values obtained from the standard. The presence of gliadin 33mer in the samples is determined directly from this curve.