alpha-2-Macroglobulin ELISA

The alpha-2-Macroglobulin ELISA is intended for the quantitative determination of α2-macroglobulin in urine, serum, and plasma.

For research use only. Not for use in diagnostic procedures.

For Laboratory Professional Use Only.

This is the RUO version of Immundiagnostik part number K 6610A.

Test Principle of the Alpha-2-Macroglobulin ELISA

In the first incubation step, the α2-macroglobulin in the samples is bound to polyclonal rabbit antibodies (in excess), which are immobilized in the microtiter wells. To remove all unbound substances, a washing step is carried out. In a second incubation step, a peroxidase-labeled anti-α2-macroglobulin antibody (POD-antibody) is added.

After another washing step to remove all unbound substances, the solid phase is incubated with the substrate, tetramethylbenzidine (TMB). An acidic stop solution is then added to stop the reaction. The color converts from blue to yellow. The intensity of the yellow color is directly proportional to the concentration of α2-macroglobulin in the sample.

A dose-response curve of the absorbance unit (optical density, OD) vs. concentration is generated, using the results obtained from the calibrators. α2-macroglobulin, present in the samples, is determined directly from this curve.