Carbonyl Protein ELISA (Plasma, cellular and extracellular liquids)

The Carbonyl Protein ELISA (Plasma, cellular and extracellular liquids) is intended for the determination of protein carbonyls in biological samples. Sample types include EDTA plasma, bronchoalveolar lavage fluid and cerebrospinal fluid, cell extracts, and other soluble protein samples.

For research use only. Not for use in diagnostic procedures.

For Laboratory Professional Use Only.

We also offer a Carbonyl Protein ELISA for use with serum and plasma samples.

Developed by Immundiagnostik AG.

Test Principle of the Carbonyl Protein ELISA

Samples containing protein are reacted with DNPH; then, the non-protein constituents and unconjugated DNPH are separated by ultracentrifugation. The proteins are adsorbed to an ELISA plate and incubated with an anti-DNPH antibody followed by antibody-linked horseradish peroxidase. Absorbances are related to a standard curve prepared with carbonylated serum albumin.

The carbonyl protein content is calculated from the estimated carbonyl concentration and the total protein content of the sample. For this reason, a parallel determination of the protein content is required.